For any and all of my personal cloning needs I run to Mr. Perbal. I have had tremendous success following his user friendly guide to cloning...
A Practical Guide to Molecular Cloning, 2nd Edition
Bernard Perbal (Centre Univ., Orsay Cedex, France)
Presents techniques tested at the Curie Institute and other leading labs and lists all commercially available enzymes, vectors, linkers, and other basic products for ready reference. Offers detailed explanation of protocols, allowing the isolation, cloning, and expression of genes from living species. Presents up-to-date techniques on sequencing, in vitro expression of cloned gene, and use of computers for study of nucleic acids, and is the only book that shows how to isolate DNA-protein complexes and new methods for mutagenesis of cloned genes. Contains 235 figures and 80 tables.
